Immunofluorescence Testing
We offer advanced Immunofluorescence (IF) testing, both; direct and indirect used for diagnosing vesiculobullous (blistering) diseases of skin, kidney diseases and autoimmune diseases respectively.
Types of Immunofluorescence Tests at NPL
Direct Immunofluorescence (DIF) Used for diagnosing vesiculobullous (blistering) skin disorders and kidney diseases.
How it works A skin or kidney biopsy is taken and stained with fluorescent antibodies that detect specific immune proteins (IgG, IgA, IgM, C3, and fibrin) deposited in tissues. Under a fluorescence microscope, these immune deposits light up, helping diagnose autoimmune conditions.
Conditions Diagnosed by DIF- Skin Disorders: Pemphigus vulgaris, Bullous Pemphigoid, Dermatitis Herpetiformis, Lupus Erythematosus etc.
- Kidney Diseases: Kidney Diseases: Lupus Nephritis, IgA Nephropathy, Anti-GBM Disease etc.
Indirect Immunofluorescence Assay (IFA) IFA is a gold-standard laboratory technique that detects specific autoantibodies in a patient’s blood sample. It is widely used for diagnosing autoimmune disorders and connective tissue diseases.
How it works The patient’s serum (blood) is mixed with specially prepared antigen-coated slides. If antibodies against the antigen are present in the blood, they bind to the slide and are detected using fluorescent markers. The fluorescence intensity indicates the presence of specific autoantibodies.
At National Path Lab (NPL), we offer a comprehensive range of Indirect Immunofluorescence Assay (IFA) tests for the diagnosis of autoimmune diseases. Our IFA panel includes essential tests such as ANA (Anti-Nuclear Antibody), Anti-dsDNA (Crithidia), ENA Combo Panel (18 Antigens), and ANCA (Anti-Neutrophil Cytoplasmic Antibodies), among others.
To further enhance diagnostic accuracy, we also provide advanced immunology-based tests, including Immunoblot and Enzyme Immunoassay (EIA), ensuring a comprehensive and precise approach to autoimmune disease detection. With this extensive portfolio, NPL proudly offers the widest range of autoimmune testing in Nepal, setting a benchmark for accuracy, reliability, and cutting-edge diagnostics.
